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rat anti integrin β1 antibody aiib2  (Developmental Studies Hybridoma Bank)


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    Developmental Studies Hybridoma Bank rat anti integrin β1 antibody aiib2
    Rat Anti Integrin β1 Antibody Aiib2, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 97/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+anti+integrin+%CE%B21+antibody+aiib2/anti-Integrin/pm41152624-273-22-27
    Average 97 stars, based on 31 article reviews
    rat anti integrin β1 antibody aiib2 - by Bioz Stars, 2026-11
    97/100 stars

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    Related Articles

    Immunofluorescence:

    Article Title: A single-chain derivative of an integrin-activating antibody potentiates organoid growth in Matrigel and collagen hydrogels.
    Article Snippet: .. Immunofluorescence analysis of apical–basal polarity and proliferation marker Ki67 P26N human colon organoids were grown for 2 days in BME ± the rat anti-integrin β1 antibody AIIB2 (Developmental Studies Hybridoma Bank (DSHB) catalog number AIIB2, Research Resource Identifier (RRID): AB_528306) at a concentration of 1 μg ml−1. .. Organoids were collected using cell recovery solution (Thermo Fisher) and fixed in 4% formaldehyde (pH 7.4) at 4 °C for 1 h. Hereafter, organoids were washed with PBS 0.1% Tween (PBST) (2% BSA).

    Marker:

    Article Title: A single-chain derivative of an integrin-activating antibody potentiates organoid growth in Matrigel and collagen hydrogels.
    Article Snippet: .. Immunofluorescence analysis of apical–basal polarity and proliferation marker Ki67 P26N human colon organoids were grown for 2 days in BME ± the rat anti-integrin β1 antibody AIIB2 (Developmental Studies Hybridoma Bank (DSHB) catalog number AIIB2, Research Resource Identifier (RRID): AB_528306) at a concentration of 1 μg ml−1. .. Organoids were collected using cell recovery solution (Thermo Fisher) and fixed in 4% formaldehyde (pH 7.4) at 4 °C for 1 h. Hereafter, organoids were washed with PBS 0.1% Tween (PBST) (2% BSA).

    Concentration Assay:

    Article Title: A single-chain derivative of an integrin-activating antibody potentiates organoid growth in Matrigel and collagen hydrogels.
    Article Snippet: .. Immunofluorescence analysis of apical–basal polarity and proliferation marker Ki67 P26N human colon organoids were grown for 2 days in BME ± the rat anti-integrin β1 antibody AIIB2 (Developmental Studies Hybridoma Bank (DSHB) catalog number AIIB2, Research Resource Identifier (RRID): AB_528306) at a concentration of 1 μg ml−1. .. Organoids were collected using cell recovery solution (Thermo Fisher) and fixed in 4% formaldehyde (pH 7.4) at 4 °C for 1 h. Hereafter, organoids were washed with PBS 0.1% Tween (PBST) (2% BSA).



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    ( A ) Representative image of spreading Cas mSc YFP-VCL MCF10A cells illustrating quantification approach. Inset shows 4 × 0.8 μm region of interest (ROI) used to quantify intensity against distance from cell edge. ( B ) Normalized intensity profiles of Cas mSc and YFP-VCL across ≥20 ROIs from several cells. Error bars indicate mean and standard error of the mean (SEM). ( C ) Heat map of normalized intensity profiles for various antigens in Cas mSc cell adhesions stained with indicated antibodies. ( D ) Normalized intensity profiles using conformation-sensitive <t>integrin</t> <t>β1</t> antibodies; <t>AIIB2,</t> total <t>integrin</t> <t>β1;</t> mAb13, bent closed (BC) and extended closed (EC) conformations; 9EG7, EC and extended open (EO) conformations; 12G10, EO conformation. ( E ) Model showing inferred progression from nascent adhesions or focal complexes containing high levels of pY410Cas, inactive integrin and pY861FAK, to focal adhesions containing low levels of Cas and high levels of active integrin, vinculin, pY397FAK, F-actin, talin, paxillin, and kindlin.
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    ( A ) Representative image of spreading Cas mSc YFP-VCL MCF10A cells illustrating quantification approach. Inset shows 4 × 0.8 μm region of interest (ROI) used to quantify intensity against distance from cell edge. ( B ) Normalized intensity profiles of Cas mSc and YFP-VCL across ≥20 ROIs from several cells. Error bars indicate mean and standard error of the mean (SEM). ( C ) Heat map of normalized intensity profiles for various antigens in Cas mSc cell adhesions stained with indicated antibodies. ( D ) Normalized intensity profiles using conformation-sensitive <t>integrin</t> <t>β1</t> antibodies; <t>AIIB2,</t> total <t>integrin</t> <t>β1;</t> mAb13, bent closed (BC) and extended closed (EC) conformations; 9EG7, EC and extended open (EO) conformations; 12G10, EO conformation. ( E ) Model showing inferred progression from nascent adhesions or focal complexes containing high levels of pY410Cas, inactive integrin and pY861FAK, to focal adhesions containing low levels of Cas and high levels of active integrin, vinculin, pY397FAK, F-actin, talin, paxillin, and kindlin.
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    ( A ) Representative image of spreading Cas mSc YFP-VCL MCF10A cells illustrating quantification approach. Inset shows 4 × 0.8 μm region of interest (ROI) used to quantify intensity against distance from cell edge. ( B ) Normalized intensity profiles of Cas mSc and YFP-VCL across ≥20 ROIs from several cells. Error bars indicate mean and standard error of the mean (SEM). ( C ) Heat map of normalized intensity profiles for various antigens in Cas mSc cell adhesions stained with indicated antibodies. ( D ) Normalized intensity profiles using conformation-sensitive <t>integrin</t> <t>β1</t> antibodies; <t>AIIB2,</t> total <t>integrin</t> <t>β1;</t> mAb13, bent closed (BC) and extended closed (EC) conformations; 9EG7, EC and extended open (EO) conformations; 12G10, EO conformation. ( E ) Model showing inferred progression from nascent adhesions or focal complexes containing high levels of pY410Cas, inactive integrin and pY861FAK, to focal adhesions containing low levels of Cas and high levels of active integrin, vinculin, pY397FAK, F-actin, talin, paxillin, and kindlin.
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    Image Search Results


    ( A ) Representative image of spreading Cas mSc YFP-VCL MCF10A cells illustrating quantification approach. Inset shows 4 × 0.8 μm region of interest (ROI) used to quantify intensity against distance from cell edge. ( B ) Normalized intensity profiles of Cas mSc and YFP-VCL across ≥20 ROIs from several cells. Error bars indicate mean and standard error of the mean (SEM). ( C ) Heat map of normalized intensity profiles for various antigens in Cas mSc cell adhesions stained with indicated antibodies. ( D ) Normalized intensity profiles using conformation-sensitive integrin β1 antibodies; AIIB2, total integrin β1; mAb13, bent closed (BC) and extended closed (EC) conformations; 9EG7, EC and extended open (EO) conformations; 12G10, EO conformation. ( E ) Model showing inferred progression from nascent adhesions or focal complexes containing high levels of pY410Cas, inactive integrin and pY861FAK, to focal adhesions containing low levels of Cas and high levels of active integrin, vinculin, pY397FAK, F-actin, talin, paxillin, and kindlin.

    Journal: eLife

    Article Title: Cas phosphorylation regulates focal adhesion assembly

    doi: 10.7554/eLife.90234

    Figure Lengend Snippet: ( A ) Representative image of spreading Cas mSc YFP-VCL MCF10A cells illustrating quantification approach. Inset shows 4 × 0.8 μm region of interest (ROI) used to quantify intensity against distance from cell edge. ( B ) Normalized intensity profiles of Cas mSc and YFP-VCL across ≥20 ROIs from several cells. Error bars indicate mean and standard error of the mean (SEM). ( C ) Heat map of normalized intensity profiles for various antigens in Cas mSc cell adhesions stained with indicated antibodies. ( D ) Normalized intensity profiles using conformation-sensitive integrin β1 antibodies; AIIB2, total integrin β1; mAb13, bent closed (BC) and extended closed (EC) conformations; 9EG7, EC and extended open (EO) conformations; 12G10, EO conformation. ( E ) Model showing inferred progression from nascent adhesions or focal complexes containing high levels of pY410Cas, inactive integrin and pY861FAK, to focal adhesions containing low levels of Cas and high levels of active integrin, vinculin, pY397FAK, F-actin, talin, paxillin, and kindlin.

    Article Snippet: Following antibodies were used: mouse anti-Cas (610271) (BD Biosciences); rabbit-phospho-Y410Cas (4011S) and phospho-Y249Cas (4014S) (Cell Signaling Technology); mouse anti-vinculin (V9131, Sigma-Aldrich); mouse anti-Crk (610035) (BD Transduction labs); mouse anti-CrkL (05-414) (Upstate); sheep anti-paxillin (AF4259, R and D Systems); rabbit anti-pY31 paxillin (44-720G, Biosource); rat anti-integrin-β1 (9EG7, 553715) and mouse anti-paxillin (610051) (BD Biosciences); rabbit anti-Talin1 (A14168-1-AP), rabbit-anti Kindlin2 (11453-1-AP), and mouse anti-FAK (66258-1-Ig) (Proteintech); rat anti-integrin-β1 (mAB13, MABT821), rabbit anti-integrin β1 (AB1952P), mouse anti-integrin β1 (12G10, MAB2247) (Millipore); rabbit-anti-integrin-β3 (A2542), rabbit-anti-integrin-αv (A2091) (abclonal); rat anti-integrin-β1 (AIIB2), mouse anti-integrin α5β1 (P8D4), and mouse anti-integrin α2β1 (P1E6) (Developmental Studies Hybridoma Bank); AlexaFluor 488 goat anti-rabbit IgG (H+L), AlexaFluor 488 goat anti-mouse IgG (H+L), AlexaFluor 647 goat anti-mouse IgG (H+L), AlexaFluor 647 goat anti-sheep IgG (H+L), and AlexaFluor 633 goat anti-rat IgG (H+L) (Invitrogen); IRDye 680RD goat anti-mouse and IRDye 800CW goat anti-rabbit (LI-COR).

    Techniques: Staining

    ( A ) Cas mSc MCF10A cell stained for pY410Cas, Total integrin β1 (AIIB2), and F-actin. ( B ) Cas mSc YFP-VCL MCF10A cell stained for pY410Cas and EC + EO integrin β1 (9EG7). ( C ) Cas mSc MCF10A cells stained for EO integrin β1 (12G10) and BC + EC integrin β1 (mAb13).

    Journal: eLife

    Article Title: Cas phosphorylation regulates focal adhesion assembly

    doi: 10.7554/eLife.90234

    Figure Lengend Snippet: ( A ) Cas mSc MCF10A cell stained for pY410Cas, Total integrin β1 (AIIB2), and F-actin. ( B ) Cas mSc YFP-VCL MCF10A cell stained for pY410Cas and EC + EO integrin β1 (9EG7). ( C ) Cas mSc MCF10A cells stained for EO integrin β1 (12G10) and BC + EC integrin β1 (mAb13).

    Article Snippet: Following antibodies were used: mouse anti-Cas (610271) (BD Biosciences); rabbit-phospho-Y410Cas (4011S) and phospho-Y249Cas (4014S) (Cell Signaling Technology); mouse anti-vinculin (V9131, Sigma-Aldrich); mouse anti-Crk (610035) (BD Transduction labs); mouse anti-CrkL (05-414) (Upstate); sheep anti-paxillin (AF4259, R and D Systems); rabbit anti-pY31 paxillin (44-720G, Biosource); rat anti-integrin-β1 (9EG7, 553715) and mouse anti-paxillin (610051) (BD Biosciences); rabbit anti-Talin1 (A14168-1-AP), rabbit-anti Kindlin2 (11453-1-AP), and mouse anti-FAK (66258-1-Ig) (Proteintech); rat anti-integrin-β1 (mAB13, MABT821), rabbit anti-integrin β1 (AB1952P), mouse anti-integrin β1 (12G10, MAB2247) (Millipore); rabbit-anti-integrin-β3 (A2542), rabbit-anti-integrin-αv (A2091) (abclonal); rat anti-integrin-β1 (AIIB2), mouse anti-integrin α5β1 (P8D4), and mouse anti-integrin α2β1 (P1E6) (Developmental Studies Hybridoma Bank); AlexaFluor 488 goat anti-rabbit IgG (H+L), AlexaFluor 488 goat anti-mouse IgG (H+L), AlexaFluor 647 goat anti-mouse IgG (H+L), AlexaFluor 647 goat anti-sheep IgG (H+L), and AlexaFluor 633 goat anti-rat IgG (H+L) (Invitrogen); IRDye 680RD goat anti-mouse and IRDye 800CW goat anti-rabbit (LI-COR).

    Techniques: Staining

    ( A ) Identification of β1 integrins required for attachment to fibronectin (FN) and COLI. Cas mSc MCF10A cells were treated with blocking antibodies α5β1 (P8D4) and α2β1 (P1E6) during spreading on FN- or COLI-coated glass for 30 min. Cells were then fixed and stained with phalloidin to visualize F-actin. Areas of 19–28 cells. ns, non-significant; ****p < 0.0001 by Kruskal–Wallis followed by Dunn’s multiple comparison test. ( B ) Organization of β1 and αv integrin adhesions on FN. Human foreskin fibroblast (HFF) cells were plated on FN for 30 min, fixed, and stained as indicated.

    Journal: eLife

    Article Title: Cas phosphorylation regulates focal adhesion assembly

    doi: 10.7554/eLife.90234

    Figure Lengend Snippet: ( A ) Identification of β1 integrins required for attachment to fibronectin (FN) and COLI. Cas mSc MCF10A cells were treated with blocking antibodies α5β1 (P8D4) and α2β1 (P1E6) during spreading on FN- or COLI-coated glass for 30 min. Cells were then fixed and stained with phalloidin to visualize F-actin. Areas of 19–28 cells. ns, non-significant; ****p < 0.0001 by Kruskal–Wallis followed by Dunn’s multiple comparison test. ( B ) Organization of β1 and αv integrin adhesions on FN. Human foreskin fibroblast (HFF) cells were plated on FN for 30 min, fixed, and stained as indicated.

    Article Snippet: Following antibodies were used: mouse anti-Cas (610271) (BD Biosciences); rabbit-phospho-Y410Cas (4011S) and phospho-Y249Cas (4014S) (Cell Signaling Technology); mouse anti-vinculin (V9131, Sigma-Aldrich); mouse anti-Crk (610035) (BD Transduction labs); mouse anti-CrkL (05-414) (Upstate); sheep anti-paxillin (AF4259, R and D Systems); rabbit anti-pY31 paxillin (44-720G, Biosource); rat anti-integrin-β1 (9EG7, 553715) and mouse anti-paxillin (610051) (BD Biosciences); rabbit anti-Talin1 (A14168-1-AP), rabbit-anti Kindlin2 (11453-1-AP), and mouse anti-FAK (66258-1-Ig) (Proteintech); rat anti-integrin-β1 (mAB13, MABT821), rabbit anti-integrin β1 (AB1952P), mouse anti-integrin β1 (12G10, MAB2247) (Millipore); rabbit-anti-integrin-β3 (A2542), rabbit-anti-integrin-αv (A2091) (abclonal); rat anti-integrin-β1 (AIIB2), mouse anti-integrin α5β1 (P8D4), and mouse anti-integrin α2β1 (P1E6) (Developmental Studies Hybridoma Bank); AlexaFluor 488 goat anti-rabbit IgG (H+L), AlexaFluor 488 goat anti-mouse IgG (H+L), AlexaFluor 647 goat anti-mouse IgG (H+L), AlexaFluor 647 goat anti-sheep IgG (H+L), and AlexaFluor 633 goat anti-rat IgG (H+L) (Invitrogen); IRDye 680RD goat anti-mouse and IRDye 800CW goat anti-rabbit (LI-COR).

    Techniques: Blocking Assay, Staining

    ( A ) Rac1 requires Cas to induce cell spreading. Images and quantification of MCF10A cells expressing EGFP-Rac1WT or -Rac1Q61L that were treated with control or Cas siRNA and fixed after 30 min of spreading. ( B, C ) Reactive oxygen species (ROS) regulates Rac1 activation, Rac1 activation, cell spreading and vinculin recruitment to Cas clusters. ( B ) FRET images and quantification of Rac1-2G MCF10A cells treated with DMSO or NADPH-dependent oxidase inhibitor diphenylamineiodonium (DPI) and fixed after 30 min of spreading. ( C ) Images and quantification of Cas mSc YFP-VCL MCF10A cells treated with DMSO or DPI. Graphs show mean cell area, mean Cas mSc and YFP-VCL intensity, and median Δ t 1/2 (VCL-Cas) from 7 to 12 cells in three biological repeats. ( D ) Inhibiting Cullins accelerates vinculin recruitment to Cas clusters. Images and quantification of Cas mSc YFP-VCL MCF10A cells treated with control or SOCS6 siRNA or with DMSO or Cullin Neddylation inhibitor MLN4924. Graphs show median Δ t 1/2 (VCL-Cas) from 8 to 12 cells in three biological repeats. All error bars represent mean and standard error of the mean (SEM) and all p values by Mann–Whitney U -tests. ( E ) Two-step model. In the first step, co-clustering of Cas with inactive integrin leads to SFK-dependent phosphorylation of Cas, recruitment of Crk/CrkL, activation of Rac1, ROS production, and positive feedback that strengthens and maintains signaling to form a nascent adhesion. Positive feedback is opposed by negative feedback resulting from CRL5 SOCS6 . In a second step, integrin β1 is activated and talin1, kindlin2, vinculin, actin, and other proteins are recruited to form a focal adhesion. The second step may be triggered by growth of the nascent adhesion to a critical size, or by decreased occupancy with Cas. See Discussion for details.

    Journal: eLife

    Article Title: Cas phosphorylation regulates focal adhesion assembly

    doi: 10.7554/eLife.90234

    Figure Lengend Snippet: ( A ) Rac1 requires Cas to induce cell spreading. Images and quantification of MCF10A cells expressing EGFP-Rac1WT or -Rac1Q61L that were treated with control or Cas siRNA and fixed after 30 min of spreading. ( B, C ) Reactive oxygen species (ROS) regulates Rac1 activation, Rac1 activation, cell spreading and vinculin recruitment to Cas clusters. ( B ) FRET images and quantification of Rac1-2G MCF10A cells treated with DMSO or NADPH-dependent oxidase inhibitor diphenylamineiodonium (DPI) and fixed after 30 min of spreading. ( C ) Images and quantification of Cas mSc YFP-VCL MCF10A cells treated with DMSO or DPI. Graphs show mean cell area, mean Cas mSc and YFP-VCL intensity, and median Δ t 1/2 (VCL-Cas) from 7 to 12 cells in three biological repeats. ( D ) Inhibiting Cullins accelerates vinculin recruitment to Cas clusters. Images and quantification of Cas mSc YFP-VCL MCF10A cells treated with control or SOCS6 siRNA or with DMSO or Cullin Neddylation inhibitor MLN4924. Graphs show median Δ t 1/2 (VCL-Cas) from 8 to 12 cells in three biological repeats. All error bars represent mean and standard error of the mean (SEM) and all p values by Mann–Whitney U -tests. ( E ) Two-step model. In the first step, co-clustering of Cas with inactive integrin leads to SFK-dependent phosphorylation of Cas, recruitment of Crk/CrkL, activation of Rac1, ROS production, and positive feedback that strengthens and maintains signaling to form a nascent adhesion. Positive feedback is opposed by negative feedback resulting from CRL5 SOCS6 . In a second step, integrin β1 is activated and talin1, kindlin2, vinculin, actin, and other proteins are recruited to form a focal adhesion. The second step may be triggered by growth of the nascent adhesion to a critical size, or by decreased occupancy with Cas. See Discussion for details.

    Article Snippet: Following antibodies were used: mouse anti-Cas (610271) (BD Biosciences); rabbit-phospho-Y410Cas (4011S) and phospho-Y249Cas (4014S) (Cell Signaling Technology); mouse anti-vinculin (V9131, Sigma-Aldrich); mouse anti-Crk (610035) (BD Transduction labs); mouse anti-CrkL (05-414) (Upstate); sheep anti-paxillin (AF4259, R and D Systems); rabbit anti-pY31 paxillin (44-720G, Biosource); rat anti-integrin-β1 (9EG7, 553715) and mouse anti-paxillin (610051) (BD Biosciences); rabbit anti-Talin1 (A14168-1-AP), rabbit-anti Kindlin2 (11453-1-AP), and mouse anti-FAK (66258-1-Ig) (Proteintech); rat anti-integrin-β1 (mAB13, MABT821), rabbit anti-integrin β1 (AB1952P), mouse anti-integrin β1 (12G10, MAB2247) (Millipore); rabbit-anti-integrin-β3 (A2542), rabbit-anti-integrin-αv (A2091) (abclonal); rat anti-integrin-β1 (AIIB2), mouse anti-integrin α5β1 (P8D4), and mouse anti-integrin α2β1 (P1E6) (Developmental Studies Hybridoma Bank); AlexaFluor 488 goat anti-rabbit IgG (H+L), AlexaFluor 488 goat anti-mouse IgG (H+L), AlexaFluor 647 goat anti-mouse IgG (H+L), AlexaFluor 647 goat anti-sheep IgG (H+L), and AlexaFluor 633 goat anti-rat IgG (H+L) (Invitrogen); IRDye 680RD goat anti-mouse and IRDye 800CW goat anti-rabbit (LI-COR).

    Techniques: Expressing, Activation Assay, MANN-WHITNEY